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Figure 4 | BMC Plant Biology

Figure 4

From: The PTI1-like kinase ZmPti1a from maize (Zea maysL.) co-localizes with callose at the plasma membrane of pollen and facilitates a competitive advantage to the male gametophyte

Figure 4

Expression of ZmPti1 genes in various tissues at different developmental stages of maize. (A) Expression of ZmPti1 genes was analyzed in the sterile flavonol-deficient whp maize line and its corresponding wild type (WT) in developing staminate spikelets 9 dba (-9), 6 dba (-6), 3 dba (-3) and at anthesis (0) in mature pollen isolated from spikelets at anthesis (pollen mature), in pollen germinated in vitro for 10 min (pollen germ.), in silks, developing kernels 20 days after pollination (kernel dev. 20 dap), in kernels 7 days after germination (kernel germ.), in roots and leaves of 7-d-old seedlings and in mature leaves. Methylene blue stained ribosomal RNA is shown as loading control. (B) Expression of ZmPti1 genes in Fusarium graminearum infected maize cobs. Pathogen infected maize cobs of line A188 were harvested over a period of four weeks after infection and of a mock infected cob (3 weeks uninfected). RNA was isolated from kernels of cobs and divided into top (T) middle (M) and bottom (B). Corresponding cobs are shown at the top of the figure. 20 μg of total RNA of each probe were utilized for Northern blotting and subsequently hybridized to probes specific to ZmPti1b and ZmPti1c, respectively. Methylene blue stained ribosomal RNA served as loading control of the gel. Transcripts of a constitutively expressed Fusarium β-tubulin gene [60] were amplified by semi-quantitative RT-PCR from the same RNA probes, blotted and hybridized to a β-tubulin specific probe. The amount of amplified cDNAs served as an indicator of the severity of pathogen infection (β-tubulin RT-PCR).

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