Figure 3

Expression of PR-2 and PR-5 and PR-8 following inoculation of apple shoots with Erwinia amylovora by three different methods. Northern hybridization of RNA preparations from young apple shoots following inoculation with E. amylovora Ea273 by piercing shoot tips with a contaminated florist's frog (Frog), slicing the two youngest unfolded leaves on either side of the mid-vein with contaminated scissors (Slice) or by snipping off the distal 1/3 of the two youngest unfolded leaves with contaminated scissors (Snip). Shoots or leaves were sampled at 6, 12, 22, 32 and 45 hours following inoculation.