PR genes of apple: identification and expression in response to elicitors and inoculation with Erwinia amylovora

Table 1 Side-by-side comparison of three putative PR proteins from apple with their respective type member.

	Apple PR-1a	PR-1 Type Member CAA29392	Apple PR-5	PR-5 Type Member CAA27548	Apple PR-8	PR-8 Type Member AAC37395
Similarity to Type Member E-value ¹	5e-54	N/A	2e-37	N/A	2e-104	N/A
Molecular Weight (Daltons)	17122	18574	25669	24552	31757	30775
Amino Acid Residues	161	168	246	226	299	292
Basic Amino Acids	8	10	14	14	16	13
Acidic Amino Acids	17	14	20	16	24	24
Hydrophobic Amino Acids	55	48	75	63	109	115
Polar Amino Acids	61	54	89	86	102	91
Isoelectric Point	4.718	5.803	4.567	5.248	4.600	4.273
Charge at pH 7.0	-8.296	-3.473	-6.405	-2.435	-7.798	-10.766
Number Amino Acids in Signal Sequence	24	30	24	25	20	25
Predicted Sub-cellular Location	Outside	Outside	Outside	Outside	Outside	Vacuole

¹ The E (expect) value is the probability that the match happened by chance. Comparison was made with mature peptide sequences (i.e. without signal sequence)
Deduced amino acid sequence statistics of PR-1a, PR-5 and PR-8 from apple were generated using the Editseq program in Lasergene^® from DNASTAR (Madison, WI, USA). Protein sequences for type members were obtained through GenBank and analyzed using the same program. Sequence similarities to type members were obtained by using the BLAST on the National Center for Biotechnology Information web site. Signal sequence and localization predictions were done by PSORT. The type members are as described by Van Loon et al. [3]

ISSN: 1471-2229