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Table 1 Comparison of qRT-PCR and microarray results for selected genes.

From: Comprehensive transcriptional profiling of NaCl-stressed Arabidopsis roots reveals novel classes of responsive genes

AGI#

annotation

microarray (log2 ratio)

qRT-PCR (log2 ratio)

  

6 h

24 h

48 h

6 h

24 h

48 h

At1g44830

AP2/ERF

4.37

5.42

4.89

6.05

6.37

6.47

At5g43650

bHLH

7.21

7.58

7.45

11.05

11.93

11.87

At4g31500

CYP83B1/SUR2

-2

-1.89

-1.43

-1.84

-1.6

-1.22

At4g26410

expressed protein

0.01

0

0.04

-0.3

-0.23

-0.27

At2g04070

MATE

3.77

5.47

5.35

3.1

4.92

5.27

At3g23250

MYB15

4.05

3.25

1.31

5.39

5.67

5

At4g01550

NAC

1.93

3.35

3.21

2.46

4.87

5.65

At2g37130

PER21

-1.73

-1.56

-0.98

-1.32

-1.4

-0.69

At3g01190

PER27

-0.37

-0.02

0.14

-2.96

-1.21

-0.18

At5g64100

PER69

-1.78

-2.69

-1.8

-1.85

-2.4

-2.2

At2g24570

WRKY17

3.74

1.94

1.16

3.84

2.15

1.64

At2g30250

WRKY25

2.59

4.04

4.49

3.49

6

6.23

At2g38470

WRKY33

4.02

4.42

3.58

4.81

5.58

4.64

At4g30280

XTH18

2.54

1.3

1.57

2.65

1.58

2.5

At3g17860

ZIM

2.28

0.65

-0.84

2.42

1.02

-0.97

  1. Genes representing a variety of expression patterns and functional categories were selected from the microarray dataset for further validation using qRT-PCR. Values shown are the mean of at least four independent measurements (microarray data), or three independent measurements (qRT-PCR).