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Table 3 Mean expression difference and 2× SD between each single gene compared to the mean expression of the other 11 listed genes.

From: An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development

2003 Pericarp

2004 Pericarp

Ranked genes*

Mean difference (C t )*

Accuracy (2× SD)

Ranked genes

Mean difference (C t )

Accuracy (2× SD)

SAND

2.70

0.343

Actin

-5.33

0.462

GAPDH (m)

-2.10

0.504

PP2A

3.10

0.496

TIP41

3.38

0.600

EF1-α

-1.86

0.604

MDH (m)

1.00

0.638

SAND

2.52

0.626

EF1-α

-1.15

0.647

GAPDH (m)

-1.02

0.630

AP47

1.37

0.719

UBC

-0.43

0.689

Actin

-5.70

0.733

AP47

0.33

0.728

PP2A

3.79

1.015

UBQ-L40

-0.49

0.889

α-Tubulin

0.39

1.039

α-Tubulin

1.23

0.892

UBC

-1.19

1.202

MDH (m)

1.82

0.925

UBQ-L40

0.96

1.213

UBQ (m)

-2.23

0.984

UBQ (m)

-3.44

1.421

TIP41

2.36

1.057

  1. Cyclophilin, EF1-α (m) and β-tubulin were excluded from analysis due to their low performances using geNorm.
  2. * Mean difference (Ct-mean Ct).
  3. Precision around the mean Ct is represented by 2× the standard deviation.