Figure 2
From: New insight into the structures and formation of anthocyanic vacuolar inclusions in flower petals
Morphology of AVIs in isolated protoplasts derived from the different epidermal cells. V, central vacuole. A. Bright field microscopy image of protoplasts isolated from the adaxial epidermis of inner petal region, showing rigid shape of the protoplasts and AVI consisting of granules and threads. B. Fluorescent microscopy image of the same protoplasts shown in A. Red color showing chloroplasts emitting red auto-fluorescence from chlorophylls. C. Bright field microscopy image of protoplasts isolated from the abaxial epidermis of the inner petal region, showing vesicle-like AVIs (arrow) in the round protoplasts. Chloroplasts, green. D. Fluorescent microscopy image of the same protoplasts shown in C. Chloroplasts revealed by the red auto-fluorescence. E. Bright field microscopy image of protoplasts isolated from the adaxial epidermis of the outer petal region, showing the presence of rod-like AVIs (arrow). F. Fluorescent microscopy image of the same protoplasts shown in E. No chloroplasts revealed.