Figure 6
From: Auxin and nitric oxide control indeterminate nodule formation
Evaluation of NO production in nodules. A. M. truncatula nodulated with S. meliloti IAA or control strain B. M. sativa nodulated with S. meliloti IAA or control strain. Upper panels: Microscopy. Bright-field images of the nodules (top) and the confocal laser scanning microscopy (CLSM) detection of endogenous NO in the same nodules (bottom, excitation at 488 nm, emission at 505–530 nm). Bars indicate 200 μm. Photographs are representative of results obtained from the analysis of nodules in three independent experiments. Lower panels: Analysis of fluorescence intensities in nodules induced by IAA and control S. meliloti strain. Results are means ± SE (from at least 15 nodules); all data are statistically significant (P < 0.05). IAA: nodules produced by the S. meliloti IAA strain. Control: nodules produced by the S. meliloti control strain.