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Figure 4 | BMC Plant Biology

Figure 4

From: Endogenous pararetroviral sequences in tomato (Solanum lycopersicum) and related species

Figure 4

Analysis of cytosine methylation in Lyc EPRV sequences. DNA of parental plants (flanking) and interspecific hybrids (central) was restricted with XbaI (lane 2 to 4 each) and either ScrFI (S) and BstNI (B) to detect CHG methylation (A, B) or Sau3aI (Sa) and NdeI (N) for asymmetric cytosine sites (C, D), the first enzyme of each pair being methylation sensitive. The first lane each contains undigested DNA (un). A, C. DNAs were hybridized to a 1.3 kb fragment of the CP/MP reading frame (E1) of a S.lycopersicum EPRV copy (Le1) and B, D. to a 580 bp fragment of the IGR (H7) of a S.habrochaites clone (Lh7).

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