Expression of SCO1. RTPCR analysis of the EF-G transcript from wild-type, sco1-1, and sco1-4 seedlings demonstrates a lower abundance of transcript level in sco1-4. The TDNA insert in sco1-4 is located 14 base-pairs upstream of the ATG start site, directly affecting its transcription rate. The genetic lesion in sco1-1 does not appear to affect the level of SCO1 mRNA, as its level appears similar to that of wild-type. The level of the loading control UBQ transcript is similar between all three samples.