Figure 2

Anthocyanin hydroxylation and expression of flavonoid 3',5'-hydroxylases. (A) Percentage of tri-hydroxylated anthocyanins among 3-monoglucoside anthocyanins; (B) evolution of anthocyanin composition from the onset of coloration to full maturity; (C) F3'5'H gene expression in four cultivars with no, low, medium and high percentage of tri-hydroxylated anthocyanins at four ripening stages. At mid-véraison green berries were analysed separately from red berries on the same cluster; (D) pattern of F3'5'H gene expression normalised to the expression level of the anthocyanin biosynthetic gene UFGT (F3'5'H /UFGT); (E) linear regression between the cumulative F3'5'H /UFGT ratio and the final percentage of tri-hydroxylated anthocyanins; (F) pattern of F3'5'H gene expression normalised to the expression level of F3'H (F3'5'H /F3'H).