Localisation of OLE-GFP/RFP in tobacco protoplasts. (a): Image of a tobacco protoplast transformed with OLE-GFP and stained with Nile red. (b): Image of a tobacco protoplast co-expressing GFP-KDEL and OLE-RFP. The scale bar corresponds to 20 μm. (c): The lipid bodies (LB), ER and cytosolic (Cyt) protein fractions recovered from tobacco protoplasts transformed with OLE-GFP were subjected to SDS-PAGE and Western blot analysis using a GFP antiserum.