Representative image of HPLE/F-YFP fluorescence distribution in the presence of oleosin. (a): Tobacco protoplasts co-expressing pG2HPLE1-YFP and OLE-RFP. (b): Tobacco protoplasts co-expressing pG2HPLF1-YFP and OLE-RFP. (c): Tobacco protoplasts co-expressing pG2HPLF2-YFP and OLE-RFP. (d): Tobacco protoplasts co-expressing pG2HPLF3-YFP and OLE-RFP. The scale bar corresponds to 20 μm. YFP (505–530 nm) fluorescence in green. (e): The lipid bodies (LB), ER and cytosolic (Cyt), chloroplastid (Chlor) protein fractions recovered from tobacco protoplasts co-expressing OLE-RFP and HPLE/F-YFP were subjected to SDS-PAGE and Western blot analysis using a GFP antiserum.