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Table 2 Summary of genes surveyed and primer sequences employed.

From: DNA sequence diversity and the origin of cultivated safflower (Carthamus tinctoriusL.; Asteraceae)

Locus Functional Annotation via BLAST Primer Sequences (Forward/Reverse)
A19 At2g21330 5'-CTAGAGAACACSGARGCTAACCG
  Putative fructose bisphosphate aldolase 5'-TGGCGAAACGRGCACCYTGTTGG
A25 At2g45740 5'-TTGCATGSTCTTATCAGTCC
  Similar to putative peroxisomal membrane protein PEX11-1 5'-GAAGABCCCATCCARCAGAAGAG
A25a - Same as A25 Forward
   5'-TCTCTCTCATGACACCATGTAAA
A25b - 5'-GCTCCACAGATCAGGCATTT
   Same as A25 Reverse
A27 At3g19900 5'-CTTGCAWTGAATGTCATGTGGAAG
  Unknown protein 5'-GCTCCCCARCATTTCA
A39 At2g28315 5'-ACTAGTTGGCATYTRATGGTAACA
  Putative glucose-6-phosphate/phosphate-tranlocator 5'-GCCRACAAAATTGAGCTGAAGATC
A39a - Same as A39 Forward
   5'-TCATGGACCAGAAATGAYGTT
A39b - 5'-AGCACCCTTCCYTACTGCAT
   Same as A39 Reverse
B7 At4g27700 5'-AGAAAAYARCTTTGTGATTCTTGATG
  Contains Rhodanese Homology Domain 5'-GAWGARCAAGCTACTATRATCTTTG
B12 At3g55800 5'-CAAGTGGCTGCAGCCATGGG
  Sedoheptulose-bisphosphatase precursor 5'-ACATCRGGMACCATTCCWCCGGTGT
B27 At4g33250 5'-AAGGCTCTTATGGCHATGCC
  Similar to eukaryotic translation initiation factor 3 subunit 11 5'-CGGTTYTTRGCWGCTTCATCCCARAACTG
B27a - Same as B27 Forward
   5'-TGATGCAAAATAGTTTGTTGGAA
B27b - 5'-TGGTGMTYCTTTTCCAATTC
   Same as B27 Reverse
  1. Primer names followed by 'a' or 'b' were designed to amplify a given locus in two parts. Functional annotations were taken from the Genbank record for each Arabidopsis locus. For the unknown protein a BLASTn search failed to yield a putative function