Time dependence of the incorporation of radiolabel from [14C]acyl-CoA into isolated plasma membranes. Plasma membranes, isolated from pea seedlings, were incubated with radiolabelled acyl-CoA for up to 80 min. Each incubation contained 25 μg plasma membrane protein. A, Distribution of radiolabel between 18:1-CoA (solid squares), free fatty acids (open squares) and phospholipids (open circles). B, Incorporation of radiolabel into PC (filled symbols) and PE (open symbols). Either [14C]18:1-CoA (solid line) or [14C]16:0-CoA (dashed line) was used as substrate. The data represent mean values ± the range of duplicates from a representative experiment.