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Figure 9 | BMC Plant Biology

Figure 9

From: LysoPC acyltransferase/PC transacylase activities in plant plasma membrane and plasma membrane-associated endoplasmic reticulum

Figure 9

Optical evidence for PAMs. Confocal laser beam microscopy of a plasma membrane fraction isolated from 2 month-old Arabidopsis thaliana, transformed with green fluorescent protein (GFP) tagged to the ER lumen [31]. The isolated plasma membranes were subjected to a repeated cycle of freezing and thawing to invert a population of the vesicles [32] and incubated with the red fluorescent probe FM4-64. The sample was observed under a 488 nm laser which excited the plasma membrane-localized probe (left image) and under a 543 nm laser which excited ER-localized GFP (middle image). To the right, these two images are merged.

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