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Table 1 Acyl incorporation into PC and PE, comparing PAM with plasma membranes and an ER-rich fraction

From: LysoPC acyltransferase/PC transacylase activities in plant plasma membrane and plasma membrane-associated endoplasmic reticulum

  Acyl incorporation from [14C]18:1-CoA (pmol·min-1·[mg protein]-1)
  PC PE
Plasma membrane   
   on its own 53 23
   + lysoPC 549 47
   +lysoPC + AgNO3 7 6
PAM-decreased plasma membrane   
   on its own 89 27
   + lysoPC 329 37
   + lysoPC + AgNO3 11 5
PAM   
   on its own 74 20
   + lysoPC 840 49
   + lysoPC + AgNO3 14 10
Enriched ER   
   on its own 91 13
   + lysoPC 899 53
   + lysoPC + AgNO3 15 8
  1. A pea shoot plasma membrane fraction was the starting material for the isolation of the PAM fraction. The PAM-decreased plasma membrane fraction represents the plasma membranes obtained after the removal of the PAM. The ER-enriched fraction is fraction number 1 from the 10-step counter current distribution (see Fig. 1). All fractions were incubated with [14C]18:1-CoA on its or in the presence of 70 μM lysoPC (acylated with 18:1), without or with 80 μM Ag NO3. Mean values are presented for 2–5 independent experiments, with standard deviations less than 10%.