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Figure 2 | BMC Plant Biology

Figure 2

From: RNAi-directed downregulation of OsBADH2 results in aroma (2-acetyl-1-pyrroline) production in rice (Oryza sativa L.)

Figure 2

Construct and molecular analysis of normal and transgenic plants. R represents the presence of RNAi construct. Schematic diagram of part of the T-DNA region of the transforming construct CMV35S-OsBADH2-RNAi is shown in (A). Inversely repeated fragments derived from 3' coding region of OsBADH2 are indicated by OsB2. (B) Semi-quantitative RT-PCR, Real-time quantitative RT-PCR analysis of OsBADH2(left) and OsBADH1(right) mRNA levels in fully expanded leaves derived from wild-type (WTa) and four OsBADH2-deficient lines (OsB2-Ra, b, c and d). Each bar represents three replications from each RNA sample. Error bars represent standard errors shown in each case.

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