Figure 2

Expression of the CBF genes and COR15a over 8 hours in the cold. Kinetics of induction of CBF1, 2 and 3 and COR15a by cold. RNA was prepared by protocol 1 (see Methods) and PCR was performed on the ABI 7900 HT platform. For each gene, eight accessions were analyzed by Q-PCR: 157 AV (Ita-0), 163 AV (Can-0), 166 AV (Cvi-0), 186 AV (Col-0), 229 AV (Rld-2), 231 AV (Rub-1), 233 AV (Sah-0), and 244 AV (WS). Sensitive accessions are in red and tolerant ones in blue. Time points shown are 10, 20, 30, 40 min, 1 h, 1.5 h, 2 h, 3 h, 4 h, 6 h, 8 h after transfer to 5°C. Average standard errors in the determination of the expression of the different genes varied between 0.16 Ct and 0.36 Ct.