Figure 5

The complementation of rack1a mutants by overexpression of RACK1 genes. (A) RT-PCR analysis of the expression of RACK1 genes in transgenic lines. The transgenic lines 2-7, 6-2, 8-3 and 25-3 are RACK1A overexpressors in rack1a-2 mutants. The transgenic lines 4-5 and 28-2 are RACK1B overexpressors in rack1a-1 mutants. The transgenic lines 4-3, 5-3, 8-3 and 9-6 are RACK1C overexpressors in rack1a-1 mutants. RT-PCR was performed at 28 cycles. The expression of ACTIN2 was used as a control. (B) The number of rosette leaves in transgenic plants overexpressing individual RACK1 gene in rack1a mutant background. The number of rosette leaves was collected from plants grown for 37 d under 14/10 h photoperiod. Shown are the averages of number of rosette leaves from at least four plants ± S.E. (C) The length of primary root in transgenic plants overexpressing individual RACK1 gene in rack1a mutant background. The length of primary roots was measured from seedlings grown for 10 d under 14/10 h photoperiod. (D) The number of lateral roots in transgenic plants overexpressing individual RACK1 gene in rack1a mutant background. The number of lateral roots was counted from seedlings grown for 11 d under 14/10 h photoperiod. Shown in (C) and (D) are the averages of at least 20 seedlings ± S.E. *, significant difference from Col, P < 0.05.