Figure 1
M. sexta damage and Pst DC3000 inoculation increase Na defensin transcripts; responses are highly attenuated in ir defensin (76 and 96) lines. Quantitative real-time PCR (qRT-PCR) was used to analyze Nadefensin transcript accumulation in WT N. attenuata plants and irdefensin (76 and 96) lines in response to continuous M. sexta feeding by first-instar larvae for 4 days or inoculation with Pseudomonas syringae pv tomato DC3000 (Pst DC3000) (1 × 105 cells/ml). Values are mean (± SE) Nadefensin transcripts from 3 replicate plants per treatment normalized to the transcript abundance of actin, which is unregulated under these conditions. Different letters indicate significant differences between genotypes damaged by M. sexta and infected by Pst DC3000.