Figure 3From: Exploring valid reference genes for gene expression studies in Brachypodium distachyonby real-time PCRPairwise variation (V) analysis of the candidate reference genes. The pairwise variation (Vn/Vn+1) was analyzed between the normalization factors NFn and NFn+1 by the geNorm software to determine the optimal number of reference genes required for qRT-PCR data normalization.Back to article page