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Figure 1 | BMC Plant Biology

Figure 1

From: Systemic resistance and lipoxygenase-related defence response induced in tomato by Pseudomonas putidastrain BTP1

Figure 1

Disease reduction following treatment of tomato with P. putida BTP1. (A): Example of tomato leaves infected by B. cinerea showing spreading lesions in control (left), but not in P. putida BTP1 treated plant (right), 96 hours after infection. (B): Reduction of disease observed in tomato plants treated with P. putida as compared with control plants after infection with B. cinerea. Bacteria were applied to tomato seeds and soil. Control plants were treated with 0.01 M MgSO4 solution. Third leaf was infected with 10 droplets of 4 ÎĽl of spore suspension containing 105 spores/ml, 0.01 M glucose, and 6.7 mM KH2PO4. Disease incidence was scored daily and was expressed in terms of percentage of B. cinerea spreading lesions. Four experiments were carried out on tomato, each with 45 leaves per treatment. The homogeneity of variances was tested and data from the different independent ISR experiments with the same set-up could not be pooled. Consequently, means from the two treatments were compared in every independent assay with one-way ANOVA by considering each leaf as experimental unit with its specific disease incidence (JMP 7 software, SAS). Bars marked with * represent statistical significant differences in infection rates of BTP1-treated plants compared to controls (P = 0.05).

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