Figure 3
Purification and analysis of the fungitoxic molecule. (A) HPLC profiles obtained for aglycone samples from control (in blue) and BTP1-treated (in red) plants at 48 hours after infection by pathogen. Samples of 20 ÎĽl (corresponding to 20 mg of leaf fresh weight) were injected on a C-18 reverse-phase column and material was eluted at various flow rates with a gradient of acetonitrile from 5% to 95%. Chromatograms represent max plots calculated from the maximum absorbance between 200 nm and 500 nm of eluting material. (B) Evolution of the quantity of the compound eluted at 47 minutes by HPLC on a C-18 reverse-phase column in P. putida BTP1-treated (â– ) and control plants (â—†) at 0, 16, 24 and 48 hours after pathogen challenge. Data are from one representative experiment (C) Determination of the molecular weight for the fungitoxic molecule accumulating in leaves of tomato plants treated by P. putida BTP1 by mass spectrometry.