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Table 2 Genetic variation in the intron containing Mal d 1 genes of linkage group 16 among ten apple cultivars.

From: Assessment of allelic diversity in intron-containing Mal d 1 genes and their association to apple allergenicity

Iso-allergen Varianta Sil. mut gDNA #b Cultivarc GenBank Accns
     GD PS IM CO JO RD FJ DS PM FS  
1.02 01 1 1 #    # #     # # * AY789240–AY789241, AY827646–AY827648
  01 2 2          *   AY789239
  01 3 3   # * * * * * *     AY827649–AY827654
  01 4 4 *       #     AY827655–AY827656
  01 5 5    #         AY827657
  01 6 6       #      AY827658
  01 7 7         +    AY827659
  09 1 8         +    AY827660
1.04 04 1 1          *   AY789242
  04 2 2 # e    # # #    # # * AY789243–AY789244, AY827661–AY827664
  05 1 3         +    AY827665
  06 1 4    #         AY827666
  07 1 5         +    AY827667
  ps1 1 6   # *    * * *     AY827668–AY827671
  ps2 1 7 *   * *    #     AY827672–AY827675
1.06A 01 1 1–10 #    # #      # * AY789249, AY827689–AY827691
  01 1 2–11          #   AY789250
  02 1 3–16          *   AY789248
  02 2 4–6   # * * * * * *     AY827692–AY827697
  02 3 5–10    #    #      AY827698–AY827699
  02 4 6–7         +    AY827700
  03 1 7–7 *       # +    AY827701–AY827703
1.06B 01 1 1 # #   # #      # * AY789251, AY827704–AY827707
  02 1 2 * *    * * # *   #   AY789252, AY827708–AY827712
  03 1 3       #    *   AY789253, AY827713
  03 2 4    #         AY827714
  04 1 5    * *        AY827715–AY827716
  05 1 6         +    AY827717
  05 2 7         +    AY827718
1.06C 01 1 1 # #   # #      # * AY789254, AY827719–AY827722
  02g 1 2          *   AY789255
  03g 1 3 * *    * * # * + #   AY789256, AY827723–AY827725
  04 1 4    #      +    AY827726
  05 1 5       #      AY827727
  06 1 6    * *        AY827728
  1. aVariant numbers of are according to the allergen list (see Table 1), those for Mal d 1.05 and Mal d 1.06 are according to Gao et al. [15].
  2. b, c, d as in Table 1
  3. e # and * For LG16, alleles that have the same symbol are in coupling phase with each other. This information was obtained by their co-segregation patterns in mapping progenies (Prima × Fiesta and Jonathan × Prima [15], and over pedigrees (Fiesta = Cox × Idared [= Jonathan × Wagner Apfel]); Ingrid Marie = Cox × open pollinated and Fuji = Ralls Janet × Delicious; Red Delicious is a colour mutant of Delicious.
  4. fps1 and ps2 of Mal d 1.04 refer to pseudo-alleles.
  5. g The allele specific markers for these alleles were derived from Table 3 of Gao et al. 2005 [15] whereby we took into account that their marker for Mal d 1.06C02 actually amplifies Mal d 1.06C03 and visa versa, thus harmonizing an inconsistency between their tables 2 and 3 in the assignment of allele and marker names to the AY789255 and AY789255 sequences.