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Table 2 Genetic variation in the intron containing Mal d 1 genes of linkage group 16 among ten apple cultivars.

From: Assessment of allelic diversity in intron-containing Mal d 1 genes and their association to apple allergenicity

Iso-allergen

Varianta

Sil. mut

gDNA #b

Cultivarc

GenBank Accns

    

GD

PS

IM

CO

JO

RD

FJ

DS

PM

FS

 

1.02

01

1

1

#

  

#

#

   

#

# *

AY789240–AY789241, AY827646–AY827648

 

01

2

2

        

*

 

AY789239

 

01

3

3

 

# *

*

*

*

*

*

   

AY827649–AY827654

 

01

4

4

*

     

#

   

AY827655–AY827656

 

01

5

5

  

#

       

AY827657

 

01

6

6

     

#

    

AY827658

 

01

7

7

       

+

  

AY827659

 

09

1

8

       

+

  

AY827660

1.04

04

1

1

        

*

 

AY789242

 

04

2

2

# e

  

#

#

#

  

#

# *

AY789243–AY789244, AY827661–AY827664

 

05

1

3

       

+

  

AY827665

 

06

1

4

  

#

       

AY827666

 

07

1

5

       

+

  

AY827667

 

ps1

1

6

 

# *

  

*

*

*

   

AY827668–AY827671

 

ps2

1

7

*

 

*

*

  

#

   

AY827672–AY827675

1.06A

01

1

1–10

#

  

#

#

    

# *

AY789249, AY827689–AY827691

 

01

1

2–11

        

#

 

AY789250

 

02

1

3–16

        

*

 

AY789248

 

02

2

4–6

 

# *

*

*

*

*

*

   

AY827692–AY827697

 

02

3

5–10

  

#

  

#

    

AY827698–AY827699

 

02

4

6–7

       

+

  

AY827700

 

03

1

7–7

*

     

#

+

  

AY827701–AY827703

1.06B

01

1

1

#

#

 

#

#

    

# *

AY789251, AY827704–AY827707

 

02

1

2

*

*

  

*

*

# *

 

#

 

AY789252, AY827708–AY827712

 

03

1

3

     

#

  

*

 

AY789253, AY827713

 

03

2

4

  

#

       

AY827714

 

04

1

5

  

*

*

      

AY827715–AY827716

 

05

1

6

       

+

  

AY827717

 

05

2

7

       

+

  

AY827718

1.06C

01

1

1

#

#

 

#

#

    

# *

AY789254, AY827719–AY827722

 

02g

1

2

        

*

 

AY789255

 

03g

1

3

*

*

  

*

*

# *

+

#

 

AY789256, AY827723–AY827725

 

04

1

4

  

#

    

+

  

AY827726

 

05

1

5

     

#

    

AY827727

 

06

1

6

  

*

*

      

AY827728

  1. aVariant numbers of are according to the allergen list (see Table 1), those for Mal d 1.05 and Mal d 1.06 are according to Gao et al. [15].
  2. b, c, d as in Table 1
  3. e # and * For LG16, alleles that have the same symbol are in coupling phase with each other. This information was obtained by their co-segregation patterns in mapping progenies (Prima × Fiesta and Jonathan × Prima [15], and over pedigrees (Fiesta = Cox × Idared [= Jonathan × Wagner Apfel]); Ingrid Marie = Cox × open pollinated and Fuji = Ralls Janet × Delicious; Red Delicious is a colour mutant of Delicious.
  4. fps1 and ps2 of Mal d 1.04 refer to pseudo-alleles.
  5. g The allele specific markers for these alleles were derived from Table 3 of Gao et al. 2005 [15] whereby we took into account that their marker for Mal d 1.06C02 actually amplifies Mal d 1.06C03 and visa versa, thus harmonizing an inconsistency between their tables 2 and 3 in the assignment of allele and marker names to the AY789255 and AY789255 sequences.
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BMC Plant Biology

ISSN: 1471-2229

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