Figure 6

Localization of At5g39790 homologue to Spinaceao leracea chloroplasts. Spinach chloroplast and cytosolic fractions were probed with antibodies to At5g39790 (-CTP). Lane 1: At5g39790 (-CTP) control, 2 ng; lane 2: 1 μg cytosolic protein; lane 3: 2 μg cytosolic protein; lane 4: 1 μg chloroplast protein, Lane 5: 2 μg chloroplast protein. The difference in molecular weight of the native protein in lanes 4 and 5, compared to that in lane 1, is due to the presence of the N-terminal purification tag in our cloned protein (see Methods).