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Figure 3 | BMC Plant Biology

Figure 3

From: The MYST histone acetyltransferases are essential for gametophyte development in Arabidopsis

Figure 3

Phenotypes induced by mutations in HAM genes. (A) Comparison of a wild-type (Col-0, top) and HAM1/ham1-1; ham2/ham2 sesquimutant silique (bottom). (B) Detail of a sesquimutant silique showing the presence of aborted ovules (arrow). Viability of mature pollen grains in thesesquimutant (D) compared to wild-type (C). Pollen grains were isolated from the anthers of opened flowers and examined by Alexander staining. Viable pollen grains were stained as purple and dead pollen grains are dark green. Bars: 100 μm. High magnification detail of pollens from wild-type (E) and the HAM1/ham1-1; ham2/ham2 mutant (F) showing the presence of 50% wild type and 50% shrunken, aborted pollen (arrows) in the mutant anthers. Bars = 50 μm. Scanning electron microscopy of pollen grains collected from wild-type (G) and HAM1/ham1-1; ham2/ham2 anthers (H). Approximately one-half of pollen grains were abnormally developed in the sesquimutant anthers (arrows). Bars: 25 μm.

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