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Table 3 Major proteins in apoplastic washing fluids of leaves of oilseed rape (Brassica napus var. napus).

From: Defence reactions in the apoplastic proteome of oilseed rape (Brassica napus var. napus) attenuate Verticillium longisporumgrowth but not disease symptoms

Spot No

Similarity (Organism) Peptide

NCBI no. (ACC)

Protein score (Threshold)

MW

Target peptide

4

α-L-arabinofuranosidase (Ath)

YVAIGNEDCGK

AFVSEYAVTGK

gi|13937191

(At3g10740)

113 (56)

75437

S (RC 2)

5

α-L-arabinofuranosidase (Rs)

GQETPGEDPLLSSK

gi|74355968

(BAE44362)

76

84750

M (RC 4)

7

α-L-arabinofuranosidase (Rs)

GQETPGEDPLLSSK

LPMTWYPQSYVEK

gi|74355968

(BAE44362)

195 (56)

84750

M (RC 4)

9

HP (pectin esterase) (Br)

TVQDAVNAAPDNNGVSK

ISEGVYEETVR

DITFQNTAGPDAHQAVAFR

gi|72384433

(homolog of At5g09770)

84 (56)

60345

C (RC 1)

27

Chitinase (Class IV) (Bn)

DLNEGDLLATPEK

AVNSMECTGGGVPSETAANR

gi|14486393

(AAK62048)

103 (56)

31219

S (RC 4)

28

Chitinase (Class IV) (Bn)

GAIQLSWNYNYGPCGR

DLNEGDLLATPEK

gi|14486393

(AAK62048)

90 (56)

31219

S (RC 4)

38

UP (basic secretory protein) (Ath)

WDQGYDVTAR

gi|15226060

(At2g15130)

81 (56)

25459

S (RC 1)

39

Germin (GER 3, GLP3) (Ath)

NPDQVTENDFAFTGLGK

gi|1755154

(At5g20630)

56 (56)

22020

S (RC 1)

40

Germin (GER 3, GLP3) (Ath)

NPDQVTENDFAFTGLGK

gi|1755154

(At5g20630)

98 (56)

22020

S (RC 1)

45

Germin (GER 3, GLP3) (Ath)

NPDQVTENDFAFTGLGK

gi|1755154

(At5g20630)

73 (45)

22020

S (RC 1)

  1. Spot numbers refer to spots in Figure 7. NCBI and accession number (ACC) of the proteins identified by comparing the peptide sequence with NCBI databank are indicated. HP = hypothetical protein, UP = unknown protein. Protein score, predicted molecular weight (MW) and predicted target peptide with reliability class (RC) are indicated. C = Chloroplast, M = Mitochondrium, S = Secretory pathway. Ath = Arabidopsis thaliana, Bj = Brassica juncea, Bn = Brassica napus, Br = Brassica rapa, Rs = Raphanus sativus