Table 1 Degenerate primer sequences and target motifs used for RGA isolation in M. acuminata Calcutta 4
Degenerate Primer | Target motif name/plant origin | Primer sequence (5' to 3')a | Author |
|---|---|---|---|
P1A (forward) | P-loop/Dicotyledon | GGIATGCCIGGIIIIGGIAARACIAC | [26] |
P1B (forward) | P-loop/Dicotyledon | GGIATGGGIGGIIIIGGIAARACIAC | [26] |
LM638 (forward) | P-loop/Monocotyledon & Dicotyledon | GGIGGIGTIGGIAAIACIAC | [23] |
P3A (reverse) | GLPL/Dicotyledon | AIITYIRIIRYIAGIGGYAAICC | [26] |
P3D (reverse) | GLPL/Dicotyledon | AIITYIRIIRYYAAIGGIAGICC | [26] |
RNBSD-rev (reverse) | RNBS-D non-TIR/Monocotyledon & Dicotyledon | GGRAAIARISHRCARTAIVIRAARC | [34] |
39F1 (forward) | Non NBS (n-terminal)/monocotyledon | TCATCAAGGACGAGCTGgarwbnatgma | This study |
1F (forward) | P-loop – GKTT/monocotyledon | GGCGGGGTGGGCaaracnacnht | This study |
P1C (forward) | P-loop – GKTT/Dicotyledon | GGICGICCIGGIIIIGGIAARACIAC | This study |
3F2 (forward) | Kinase 2/monocotyledon | GAGGTACTTCCTGGTGCTGgaygayrtbtgg | This study |
2F (forward) | RNBS-B/monocotyledon | AACGGCTGCAGGATCATGrtbachachmg | This study |
1R1 (reverse) | P-loop/monocotyledon | CGTGCTGGGCCAGGgtngtyttncc | This study |
P3B (reverse) | GLPL/Dicotyledon | AIITYIRIIRYIAGIGGIAGICC | This study |
13R1 (reverse) | LRR/monocotyledon | CGGCCAAGTCGTGCAyvakrtcrtgca | This study |
11R1 (reverse) | LRR/monocotyledon | TCAGCTTGCCGATCCACtydggsagbyt | This study |