Global gene expression analysis of apple fruit development from the floral bud to ripe fruit

BMC Plant Biology

Table 4 Enzymes involved in Starch metabolism

Enzyme	EC #	A. thaliana gene	Genbank acc.^a	expect value^b	qPCR vs array^c	Localisation
Sucrose synthase	2.4.1.13	At3g43190	EB144194	0	+	plastidic
		At4g02280	CN897963	0	++	unknown
		At5g20830
		At5g37180
		At5g49190
UDP-glucose pyrophosphorylase	2.7.7.9	At5g17310	EG631379	1e-173	+++	endomembrane system
Starch synthase	2.4.1.21	At1g32900	EE663720	0	-	plastidic
		At3g01180	EB121923	0	+++	plastidic
ADP-glucose phosphorylase	2.7.7.27	At1g27680	CN884033	1e-167	+++	plastidic
		At2g21590
		At4g39210
		At5g19220
		At5g48300
		At1g05610
Starch phosphorylase	2.4.1.1	At3g29320	EE663644	0	-	plastidic
		At3g46970	EB108842	1e-115	-	unknown
Sucrose-phosphate synthase	2.4.1.14	At5g20280	EB112628	0	++	unknown
		At1g04920	EB123469	0	++	unknown
		At5g11110
		At4g10120
β-amylase	3.2.1.2	At4g15210	EB114557	1e-116	+++	plastidic
		At4g17090	EG631202	1e-104	-	plastidic
α-glucosidase	3.2.1.20	At3g45940	EE663791	0	+++	endomembrane system
		At5g11720	EE663790	0	-	endomembrane system
		At5g63840
Sucrose phosphatase	3.1.3.24	At2g35840	EB156512	0	+++	cytoplasm

Starch metabolism genes were identified and the expression of putative apple starch metabolism genes confirmed by qRT-PCR.
a The representative EST on the array is shown for the best apple gene match to the Arabidopsis gene.
b The significance of the BLAST comparison between the Arabidopsis gene and the best apple gene.
c The degree of correspondence between pattern of gene expression by microarray and the pattern by qPCR. - = no correspondence; + = more than two points of divergence; ++ = good correspondence but some differences; +++ = strong correspondence

ISSN: 1471-2229