Figure 4

Completely conserved ExonS event in plant enoyl-CoA hydratase/isomerase genes. A: same-species and cross-species EST alignments in Mt gene locus AC145499_47. Filled boxes and arrows indicate exons, and lines indicate introns. Green open or filled boxes indicate exons skipped or retained in certain ESTs. The top black scale indicates coordinates for the gene locus on BAC (AC145499). The blue bar represents the IMGAG annotated gene model, with the green triangle representing the protein translation start codon and the red triangle representing the stop codon. Red bars represent individual same species EST alignments. Purple bars represent Lj ESTs, dark yellow bars represent soybean ESTs, and gray bars represent ESTs from other legume species. B. Multiple sequence alignments of the mutual exclusive exons. E3 indicates the Exon 3 and E4 indicates the Exon 4. At2E3 refers to the exon in the second copy of At gene (At4g13360). Amino acids encoded by Mt sequences are list at the top of sequence alignment. Degenerate positions (change in nucleotide will not change amino acids) which are conserved in all exons are highlighted in colors. C. EST alignment in the second copy of At gene (At4g13360). Only exon E3 exists in this gene and no ExonS can be detected. D, E. EST alignment in At and Os genes where the ExonS pattern is completely conserved.