Figure 3

Association of ATM1 with BFA bodies in specific cells. Seedlings were treated with BFA and FM4-64 and image acquisition was performed with a confocal microscope. A-B. Cells with GFP-ATM1(IQ-tail) organized in dots (arrows). A. GFP-ATM1. B. BFA bodies formed in these cells, shown by FM4-64. Note that the dotted pattern is not disrupted by the treatment (arrows). C. Overlay of A and B. Scale bar 10 μm. D-F Showing cells near the root cap where ATM1 is found in BFA bodies. D. GFP-ATM1, E. BFA bodies stained by FM4-64, F. overlay of D and E. Scale bar 10 μm. All images in this figure are composed of one optic section.