Figure 2

Microscopic analysis of P. medicaginis infection of M. truncatula. Leaves were spot inoculated with P. medicaginis OMT5 and hyphae visualised with trypan blue (A-C) or DiOC6 (D, E) at 24 and 48 h post infection. (A) P. medicaginis entering a stomata of SA3054. (B, C) build-up of H₂O₂ around the sites of penetration in SA27063. (D, E) Autofluorescence of phenolic compounds around the infection site in both SA27063 (D) and SA3054 (E). H₂O₂ = hydrogen peroxide; ifh = infection hyphae; p = point of penetration; sp = spore; st = stomata.