Confocal laser-scanning images of GFP expression patterns during ovule and seed development in enhancer-trap lines KS110, KS151, and KS149. (A) to (F) GFP expression in line KS110. (A), (B) Ovule development: GFP is expressed in the abaxial layer of the outer ovule integument and in a subset of cells on the gynoapical side of the funiculus region (arrow). (C) to (F) Seed development: GFP is expressed throughout the o.i.2. (G) to (L) GFP expression in line KS151. (G) No GFP expression is seen during early ovule development. (H) After fertilization, GFP can be seen at the micropylar end of both integuments in the abaxial cell layers. (I) to (L) Late seed development: GFP is expressed on the micropylar end of the i.i.2 and throughout the o.i.2. (M) to (R) GFP expression in line KS149. (M), (N) GFP is initially expressed only in the o.i.2. (O) to (R) During seed development, GFP expression is also seen in the o.i.1. The arrow in (O) shows beginning of expression in the o.i.1. (E), (K), and (Q) are overlay projection images of (D), (J), and (P), respectively. (F), (L), and (R) show details of outer integument expression Scalebars: 20 μm.