Seed coat phenotype of KNAT1 misexpressing plants. Confocal images of seed coats stained with safranin O. (A) Wild type seed coat. (B) Example of seed coats of a KNAT1 over-expressing line. Images are overlay projections. Arrows show the positioning of the integument closure. Scalebars: 100 μm. (C) Average area of cells in the outer seed coat of mature Arabidopsis seed of different transgenic lines and different ecotypes. Shown are data for ecotypes C24 and Landsberg erecta (Ler), for different KNAT1 overexpression lines (35SK1-xx) in C24 and Ler backgrounds, for a KNAT5 overexpression line (35SK5-71) in C24 background, and for brevipedicellus (bp) in Ler background. For each data point 25 – 30 cells of 3 different seeds were measured. Error bars show standard error of the means.