Figure 4
From: In silicoidentification of conserved microRNAs in large number of diverse plant species
Small RNA blot analysis of newly identified small RNAs in Brassica . An amount of 20 μg of low-molecular-weight RNA used for northern analysis. Antisense oligonucleotide probes were designed for the Arabidopsis miRNAs to detect their expression in Brassica oleracea (Bo) and Brassica rapa (Br) seedlings. Radiolabeled antisense oligonucleotide probes were used for detection of miRNAs (A) or radiolabeled antisense LNA-probes for detection of small-85 and small-87 (B). Blots were re-probed with U6, which served as a loading control.