Figure 5

An auxin-responsive reporter is reduced in ibr5. (A) 8-day-old light-grown Col-0 (Wt) and ibr5-1 seedlings carrying the DR5:GUS construct [13, 37] were mock treated or treated with 1 μM IAA, 10 μM IAA, 10 μM NAA, or 10 μM 2,4-D for 2 hours, then stained for GUS activity. Scale bar = 0.5 mm. (B) 5-day-old dark-grown Col-0 (Wt) and ibr5-1 seedlings carrying the DR5:GUS construct [13, 37] were mock treated or treated with 1 μM IAA, 10 μM IAA, 10 μM NAA, or 10 μM 2,4-D for 2 hours, then stained for GUS activity. Scale bar = 1 mm.