A spatial dissection of the Arabidopsis floral transcriptome by MPSS

BMC Plant Biology

Table 1 Inflorescence transcriptome diversity, as measured by MPSS

Floral Strain or Tissue	Total Distinct Expressed Genes^a	Inflorescence Enriched (%)^b	Undetected within Leaf or Root (%)^c
wild type	14,338	49.46	12.63
apetala 1	14,918	47.51	13.38
apetala 3	14,431	51.74	10.61
agamous	12,026	50.19	7.93
sup/ap1	12,505	55.53	10.97
Ovule	10,897	58.97	9.65

^a MPSS signatures within exons, within 500 bp of an annotated gene, in an intron, and overlapping an exon-intron splice boundary were quantified and "insignificant" signatures in only one sequencing library and those signatures corresponding to multiple regions of the genome were removed to demarcate gene expression.
^b Enrichment within the inflorescence signifies only those genes expressed at >4 TPM within the inflorescence. Moreover, only those genes whose expression in root or leaf tissue was less than expression within the mutant inflorescence were considered inflorescence-enriched.
^c Genes with TPM >0 in MPSS root and leaf libraries were subtracted from each floral library to assess inflorescence-specificity.

ISSN: 1471-2229