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Table 1 Redox regulation of Rap2.4 transcriptional activation

From: The redox-sensitive transcription factor Rap2.4a controls nuclear expression of 2-Cys peroxiredoxin A and other chloroplast antioxidant enzymes

 

Control

1 mM H2O2

1 mM DTT

1 mM Asc

35S:Rap2.4a

180 ± 30 %*

279 ± 56 %*

67 ± 11 %*

63 ± 10 %*

35S:CFP

100 ± 24 %

137 ± 18 %*

68 ± 9 %*

61 ± 11 %*

Rap2.4a/CFP

180 ± 29 %*

203 ± 38 %*

99 ± 10 %

103 ± 11 %

  1. Relative luciferase activity (mean ± SD) in mesophyll protoplasts from transgenic Arabidopsis expressing luciferase under control of the 2CPA promoter, 2 h after transfection with either 35S:Rap2.4a or 35S:CFP, and 1.5 h after application of 1 mM H2O2, DTT and ascorbate, respectively. Significance of difference (P > 0.01; Student's T-Test; n = 72) is indicated by asterisks.