Skip to main content

Table 2 Redox regulation of the DNA-binding affinity of Rap2.4

From: The redox-sensitive transcription factor Rap2.4a controls nuclear expression of 2-Cys peroxiredoxin A and other chloroplast antioxidant enzymes

Control

5 mM H2O2

5 mM DTT

100 ± 18 %

309 ± 87 % *

728 ± 144 % *

  1. The relative amount of unbound DNA (mean ± SD) after incubation of 2 μg Rap2.4a with 100 pmol F5 in 0.5 Ă— TBE of 0.5 Ă— TBE supplemented with 5 mM DTT or H2O2, gel extraction of unbound F5, PCR amplification and fluorometric detection on agarose gels. Significance of difference is indicated by asterisks (P > 0.05; Student's T-Test; n = 5)
Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us