Figure 2

Subcellular localization of SAPK4 -protein. (A) Nuclear localization of SAPK4-GFP fusion protein in onion epidermal cells. The arrow points to the nucleus. (B) The GFP-derived fluorescence signal of SAPK4-GFP fusion protein was merged with a light microscopic image of the transformed onion epidermal cell. (C) Onion epidermal cells transformed with a translational construct of GFP as a positive control showed localization throughout the cell with strongest signals in cytoplasm and nucleus. (D) Onion epidermal cells transformed with the empty vector as a background control. (E) Cytoplasmic localization of SAPK4-GFP fusion proteins in protoplasts of A. thaliana. nu – nucleus, ch – chloroplast, cy – cytoplasm.