- Research article
- Open Access
Constitutive Expressor of Pathogenesis-Related Genes5affects cell wall biogenesis and trichome development
BMC Plant Biology volume 8, Article number: 58 (2008)
The Arabidopsis thaliana CONSTITUTIVE EXPRESSOR OF PATHOGENESIS-RELATED GENES5 (CPR5) gene has been previously implicated in disease resistance, cell proliferation, cell death, and sugar sensing, and encodes a putative membrane protein of unknown biochemical function. Trichome development is also affected in cpr5 plants, which have leaf trichomes that are reduced in size and branch number.
In the work presented here, the role of CPR5 in trichome development was examined. Trichomes on cpr5 mutants had reduced birefringence, suggesting a difference in cell wall structure between cpr5 and wild-type trichomes. Consistent with this, leaf cell walls of cpr5 plants contained significantly less paracrystalline cellulose and had an altered wall carbohydrate composition. We also found that the effects of cpr5 on trichome size and endoreplication of trichome nuclear DNA were epistatic to the effects of mutations in triptychon (try) or overexpression of GLABRA3, indicating that these trichome developmental regulators are dependant on CPR5 function for their effects on trichome expansion and endoreplication.
Our results suggest that CPR5 is unlikely to be a specific regulator of pathogen response pathways or senescence, but rather functions either in cell wall biogenesis or in multiple cell signaling or transcription response pathways.
Mutations in the CONSTITUTIVE EXPRESSOR OF PATHOGENESIS-RELATED GENES5 (CPR5) gene of Arabidopsis thaliana are highly pleiotropic, affecting pathogen responses, cell proliferation, cell expansion, and senescence. The gene was initially identified based on the constitutive pathogen response phenotype of the mutants [1, 2], and appears to act just downstream of pathogen recognition and upstream of salicylic acid in NPR1-dependent disease resistance . In addition, Boch and co-workers  showed that CPR5 activates pathogenesis-related (PR) gene expression in the RPS2-mediated pathway and not the RPM1-mediated pathway. However, CPR5 appears to play a broader role in plant growth and development as well, because cpr5 mutants exhibit defects in cell proliferation and cell expansion, and the gene has been hypothesized to play a role in programmed cell death . In addition, cpr5 mutants are hyper-responsive to glucose and sucrose and prematurely accumulate senescence-regulated transcripts . The CPR5 gene encodes a putative membrane protein with five putative transmembrane domains at the carboxy-terminus, a putative bipartite nuclear localization signal at the amino-terminus, and no sequence similarity to other known proteins [3, 4].
In contrast to other constitutive pathogen response mutants, cpr5 mutations affect trichome morphology. The trichomes on Arabidopsis leaves are specialized single cells that project from the epidermis, and in wild-type they have an unusual branched shape. In addition, wild-type trichomes replicate their DNA without division during development in a process called endoreplication or endoreduplication, reaching nuclear DNA levels of 16C-32C [5, 6]. Trichomes of cpr5 mutants are smaller and less branched than those of wild-type, and have a lower nuclear DNA content . This trichome phenotype suggests that, unlike other constitutive pathogen response mutants, CPR5 may play a more specific role in trichome development.
Arabidopsis trichomes are used as a model of plant cell differentiation and cell biology [7, 8], and the control of early trichome development is well-understood. Initiation of trichome development requires a transcription factor complex consisting of the basic-helix-loop-helix transcription factor GLABRA3 (GL3), the Myb transcription factor GLABRA1 (GL1), and the WD-repeat protein TRANSPARENT TESTA GLABRA (TTG). Mutations in these genes result either in the absence of trichomes, or in reduced numbers of trichomes, and interactions among these proteins have been demonstrated in yeast. The TRIPTYCHON (TRY) protein acts as a negative regulator of trichome development, and is thought to prevent neighboring cells from developing as trichomes by diffusing to neighboring cells via plasmodesmata and inhibiting trichome development in a classic lateral inhibition mechanism. TRY has a Myb DNA-binding domain, but lacks a transcription activation domain, and can bind to GL3 in yeast, suggesting that it directly inhibits function of the GL1/GL3/TTG complex in cells neighboring a developing trichome .
Several mutants affect endoreplication levels in trichomes, and these mutants reveal that nuclear DNA content, trichome size, and trichome branching are highly correlated, with mutants resulting in higher DNA contents generally having trichomes that are larger and more branched [5, 10, 11]. Among the genes that control the degree of trichome expansion and endoreplication are the trichome cell fate regulators themselves. Endoreplication is reduced in gl3 loss-of-function mutants, and these mutants have smaller trichomes with reduced branching, while try mutants have increased levels of trichome endoreplication, and increase trichome size and branching . Trichomes of plants containing the gain-of-function gl3-sst allele also have large, extra-branched trichomes with enlarged nuclei indicative of an increased DNA content . These observations indicate that GL3 is required for continued trichome development beyond initiation, and that TRY acts within developing trichomes to limit the extent of expansion and endoreplication, in addition to its role in preventing neighboring cells from developing as trichomes.
To gain insight into the function of CPR5, we examined the role of this gene in the context of the well-understood pathway for trichome development. Here, we show that cpr5 mutants have altered cell walls with a reduced cellulose content in leaves as well as in trichomes, a previously unrecognized aspect of the phenotype. We also find that cpr5 mutations are epistatic to the extra expansion of trichome cells conditioned by either GL3 gain-of function and try loss-of-function. The cpr5 mutations also increase the number of adjacent trichomes formed due to failure of lateral inhibition signaling in try mutants. Our work indicates that the pleiotropy of cpr5 mutants is due to a primary role for the gene product in a general cellular process such as cell wall biogenesis or integrity that impinges on many cellular pathways, rather than a specific role in pathogen response signaling or senescence.
Two recessive alleles were used in this study, cpr5-1  and cpr5-2 . As described previously by others, the overall size of cpr5 mutant plants was smaller than wild-type, cotyledons of cpr5 plants senesce sooner than those of wild-type, lesions are present on cpr5 rosette leaves, and both leaf epidermal cell size and cell number were greatly reduced in comparison with wild-type [1–4]. For all aspects of the phenotype, cpr5-1 plants have a more severe mutant phenotype than cpr5-2 mutant plants. The cpr5-1 mutation is a missense mutation in the fourth exon (G420D), and the cpr5-2 mutation creates a premature stop in the fourth exon at codon 477. Of greatest relevance to this work, trichome branching and size were reduced in plants homozygous for either cpr5 allele (Figure 1A, B, C; Table 1). For cpr5-1 homozygotes, more than 60% of the trichomes were unbranched, essentially the same fraction of unbranched trichomes that was reported by Kirik et al.  for the strong cpr5-T1 allele, indicating that cpr5-1 results in a loss of function comparable to that of the strongest characterized alleles.
cpr5mutants have an altered cell wall
Trichomes of cpr5 mutants were more transparent than those of wild-type, and appeared glassy, suggesting that the trichome cell wall of the mutants differed from wild-type trichome cell walls. One readily observable property of plant cell walls is the birefringence they exhibit in polarized light due to the presence of paracrystalline cellulose, a major component of plant cell walls. Paracrystalline cellulose contributes to the high degree of birefringence observed in wild-type trichome cell walls . This birefringence depends on the orientation of the sample relative to the plane of polarization of the illuminating light.
We examined wild-type and cpr5 trichomes by polarized light microscopy. As expected, wild-type trichomes were highly birefringent, indicated by transmission of light when a trichome branch was oriented appropriately relative to the plane of polarization (Figure 2A), whereas cpr5-1 trichomes showed little detectable birefringence (Figure 2C), and cpr5-2 trichomes exhibited reduced birefringence (Figure 2E). Quantitative comparison between the maximum amount of transmitted light (Figure 2A, C, E) and minimum amount of transmitted light (Figure 2B, D, F) as the specimen was rotated revealed a 36.0 ± 7.8-fold difference for wild-type trichomes, a 2.0 ± 2.0-fold difference for cpr5-1 trichomes, and a 16.0 ± 11.0-fold difference for cpr5-2 trichomes.
Several other mutants with transparent "glassy" trichomes have been described [5, 12]. For the best characterized of these, trichome birefringence (tbr), reduced birefringence of trichome cell walls was associated with reduced paracrystalline cellulose in leaves . As determined by the chemical method of Updegraff , there was significantly less paracrystalline cellulose in the walls of cpr5-1 rosette leaves (p < 0.002), with walls of the mutant containing approximately 38% of the paracrystalline cellulose of wild-type walls (Figure 3A). The cell wall monosaccharide composition of rosette leaf cell walls was also determined. No qualitative differences were found, though small increases in xylose (p < 0.05) and arabinose (p < 0.01) were observed in cpr5-1 relative to wild-type (Fig. 3B). The thickness of cell walls between adjoining epidermal cells of cpr5-1 and wild-type was directly examined by TEM. The cpr5-1 mutant was found to have slightly but significantly thinner walls than wild-type (p < 0.01, Fig. 4).
Genetic interactions between cpr5and genes involved in trichome initiation
To gain further insight into the role of CPR5 in trichome development, cpr5-1 and cpr5-2 mutants were crossed with mutant plants for the trichome developmental regulator try, which has effects on trichome size, branching, and endoreplication opposite those of cpr5 mutants. The TRY gene encodes a Myb protein that acts as an inhibitor of trichome development. The mutation in the try-JC allele used here results in a protein truncated in the middle of the conserved Myb DNA-domain , and is phenotypically a strong loss-of-function allele.
Trichomes of try-JC plants are larger and more branched than those of wild-type plants, and highly birefringent (Fig. 1A, D). In contrast, the trichomes of double mutant cpr5-1 try-JC and cpr5-2 try-JC plants are similar in size to those of corresponding cpr5 allele (Fig. 1B, C, E, F), indicating that cpr5 is epistatic to try with regard to trichome size. The reduced DNA content of cpr5 trichome nuclei is also clearly epistatic to the increased DNA content of try-JC trichome nuclei (Fig. 5A, Table 2).
Unlike the trichomes of wild-type plants, try trichomes often occurred in clusters of immediately adjacent trichomes due to failure of lateral inhibition signaling (Fig. 1D). Like wild-type trichomes, trichomes on cpr5 mutant leaves only rarely occurred in clusters (Fig. 1A, B, C, Table 3). Trichomes of the cpr5 try double mutants frequently occur in clusters (Fig. 1E, F), indicating that cpr5 was not epistatic to this aspect of the try phenotype. However, closer inspection revealed an unexpected synthetic genetic interaction whereby the cpr5 mutations increased the number of trichomes in each trichome cluster above that seen for try-JC alone (Table 3). The percentage of trichomes in clusters on cpr5-1 try-JC and cpr5-2 try-JC leaves was approximately double the percentage on try-JC leaves (Table 3). This difference was due primarily to an increase in the number of trichomes in each cluster for each of the cpr5 try double mutants relative to try-JC (p < 0.001 for the comparison of either double mutant with try-JC), which averaged nearly three trichomes per cluster, compared to two trichomes per cluster in the try-JC single mutant (Table 3, p < 0.001 for the comparison of either double mutant with try-JC, and Fig. 1D,E,F).
Plants expressing the trichome developmental regulator GL3 from the strong and relatively trichome-specific GL2 promoter (proGL2:GL3) had large, highly birefringent trichomes with increased branching (Fig. 6C), similar to try-JC trichomes. Plants of the genotype cpr5-1 proGL2:GL3 have small trichomes similar to those of cpr5-1 plants (Fig. 6B,D), indicating that cpr5 is also epistatic to the increased trichome size conditioned by this construct. Wild-type plants containing the proGL2:GL3 construct also endoreplicate trichome nuclear DNA to very high levels, on the order of ten times that of wild-type (Fig. 5B, Table 3). The cpr5-1 proGL2:GL3 double mutant has a DNA content similar to that of the cpr5-1 single mutant, indicating that cpr5 is epistatic to proGL2:GL3 with regard to the degree of endoreplication (Fig. 5B, Table 4).
Discussion and Conclusion
CPR5 has been variously proposed to play specific roles in pathogen response signaling , senescence , and cell proliferation and cell death . The work presented here identifies a previously unrecognized cell wall defect in cpr5 mutants, resulting in a deficit of paracrystalline cellulose. At the same time, the epistasis of the cpr5 phenotype in genetic interactions with try and proGL2:GL3 indicates that CPR5 function is necessary for the increased cell expansion and endoreplication conditioned by loss of TRY function or by over-expression of GL3. These two genes encode transcription factors that play opposing roles in trichome development. Unexpectedly, cpr5 mutations also appear to enhance the lateral inhibition signaling defect of try mutants that normally prevents trichomes from forming adjacent to one another (Table 2).
It is difficult to reconcile our results with the specific roles that have been proposed by others as the most fundamental function of the CPR5 gene product, such as pathogen response signaling or endoreplication and programmed cell death. No other constitutive pathogen response mutants have been reported to affect trichomes, and we have observed no trichome defects on examining constitutive expressor of pathogenesis-related genes1, nonexpressor of PR genes1 (npr1), accelerated cell death2 (acd2), and accelerated cell death6 (acd6), and these mutants appeared to have normal birefringent trichome cell walls (J. C. Larkin, unpublished observations). Similarly, gl3 loss-of-function mutants, which have reduced endoreplication, produce normally birefringent trichome walls , and trichome walls of plants expressing proGL2:ICK1/KRP1, a construct that induces programmed cell death in trichomes , also appear to be normal (R. Kasili and J. C. Larkin, unpublished observations). It thus seems likely that the CPR5 gene product is involved in some general process that is indirectly necessary for trichome cell expansion, pathogen response signaling, and suppressing premature senescence and programmed cell death, rather than being a specialized component of any one of these processes.
An attractive locus of action for the CPR5 gene product suggested by our data is the cell wall itself. The cell wall is directly involved in several processes related to the cpr5 mutant phenotype, including both cell expansion and pathogen responses . Recently, a reduction of the rhamnogalacturonan II component of tobacco cell walls by Virus-induced Gene Silencing (VIGS) of a UDP-D-apiose/UDP-D-xylose synthase gene was shown to result in dwarfing of plants, induction of several pathogen-response genes, production of reactive oxygen species, and cell death . A cev1 mutant of Arabidopsis, which has a mutation in the CES3A cellulose synthase gene, was also shown to result in constitutive expression of pathogen response genes and to have enhanced pathogen resistance . This mutant was originally identified as an activator of jasmonic acid signaling pathways, and overproduces jasmonic acid and ethylene. Furthermore, a mutation in another cellulose synthase gene, rsw1, also results in activation of jasmonic acid signaling, and cellulose biosynthesis inhibitors can mimic the cev1 mutant phenotype in wild-type plants, including the activation of pathogen response genes . Jasmonic acid-dependant pathogen response pathways are known to be activated in cpr5 mutants [1, 19]. And, not surprisingly, mutations in cellulose synthase genes can affect cell morphology and expansion [20, 21]. Indeed, the cell expansion defects seen in cpr5 mutants are comparable in severity to those seen in cellulose synthase mutants [3, 22]
Taken together, these results demonstrate that defects in the cell wall itself can lead to many of the specific aspects of the cpr5 mutant phenotype, including the constitutive pathogen response signaling for which it is named. One aspect of the phenotype that is less obviously coupled to the cell wall is the reduced endoreplication seen in cpr5 . However, the degree of endoreplication is often strongly correlated with cell size , and it is possible that limitations on cell expansion have a feedback effect on endoreplication.
An alternative model to explain the extreme pleiotropy of the cpr5 mutant phenotype that is the CPR5 protein may be required directly for the function of multiple transcription factors involved in a wide range of distinct processes. Our observation that cpr5 is epistatic to the phenotypes conditioned by try loss-of-function or GL3 overexpression indicates that CPR5 function is required for the cell expansion and increased endoreplication conditioned by these two transcription factors. The CPR5 gene product is predicted to be a Type IIIa membrane protein with five transmembrane domains and a cytoplasmic N-terminus. This N-terminal domain contains a bipartite nuclear localization sequence (NLS), and it has been proposed that the protein may be involved in a signaling cascade in which the cytoplasmic domain is proteolytically cleaved and transported into the nucleus , a signaling process for which there is substantial precedent [23, 24]. An alternative is suggested by recent work demonstrating that some membrane proteins in yeast that localize to the inner nuclear membrane are targeted to this membrane via an NLS and use an karyopherin-dependant pathway to enter the nucleus . In this case, the full-length CPR5 protein might be directly required in the nucleus for function of multiple transcription factors.
Either of these models, a primary role for CPR5 in cell wall biogenesis or a primary role for CPR5 in regulation of nuclear transcription, can provide an explanation for the reduced lateral inhibition (i.e., increased trichome cluster size) seen in the cpr5 try double mutants (Table 3). Altered cell wall structure could reduce transmission of the inhibitory signal by reducing intercellular transport of the functionally redundant members of the TRY protein family, CPC, ETC1, and ETC2, perhaps by altering plasmodesmata. Alternatively, if CPR5 is needed for function of the GL3-TTG-GL1 transcriptional activation complex in the nucleus, reduced activity of this complex might result in inefficient upregulation of these TRY homologs in developing trichomes, reducing the degree of inhibition of trichome development in neighboring cells.
It is obvious that testing these models will require biochemical analysis of localization and function of the CPR5 protein. Perhaps because it is a membrane protein, little progress has been reported on this front, and our own attempts in this regard have not been fruitful. For example, fluorescent protein fusions to the CPR5 coding sequence were generated that fully complemented the cpr5 mutant phenotype, but no fluorescence was detected in any of the transgenic lines (V. Kirik, unpublished observations). Nevertheless, the work presented here suggests that the cell wall may be a unifying locus for CPR5 function, and will provide guidance for further studies. The important role of CPR5 in multiple essential aspects of plant growth and development merits further work to unravel the mechanism of CPR5 function.
Plant materials and growth conditions
Plants were grown under constant illumination as described previously. All alleles originated in the Columbia ecotype, which was used as the wild-type for these studies, and all alleles had been backcrossed to Columbia at least twice prior to use in this work. The cpr5-1 allele was obtained from Dr. Xinnian Dong ; cpr5-2 derives from our previous work . The try-JC allele has been described previously [14, 26]; in the work of Schellman et al. , it is mis-labeled as the "try-5C" allele. The identity of the cpr5 try-JC double mutants was confirmed by the failure of the double mutants to complement either parent mutant after crossing. The early senescence of cpr5 cotyledons was maintained in the double mutants and aided in identifying them. The proGL2:GL3 construct has been previously described .
For carbohydrate analysis, uncrowded plants just prior to bolting were placed in the dark for 24–48 hours to reduce the amount of starch in the leaves. The paracrystaline cellulose content of ethanol-washed cell walls (three washes of 70% ethanol at 70°C) of rosette leaves was determined by the method of Updegraff , using cellulose from Sigma as the standard.
For analysis of non-cellulosic wall monosaccharides, cell wall material was prepared by grinding leaf tissue in 80% ethanol, washing residue with 80% ethanol, then 100% ethanol, treating residue for 30 minutes with methanol:chloroform (1:1 v/v), washing residue with acetone, and air drying the residue. Further preparation and monosaccharide composition analysis was provided by the Complex Carbohydrate Research Center at the University of Georgia, Athens, GA. This included hydrolysis using freshly prepared 1 M methanolic-HCl for 16 hours at 80°C and derivatization of the released sugars with Tri-Sil. The samples were analyzed by GC using a Supelco column. Myo-inositol was added as an internal standard.
Samples fixed in FAA (3.7% formaldehyde, 50% ethanol, 5% acetic acid) were prepared for scanning electron microscopy by standard methods, as described previously . For TEM, leaves were fixed in 2% glutaraldehude and 1% paraformaldehyde in 0.2 M cacodylate buffer (pH 7.4) at room temperature for 2 hours, then rinsed with 0.1 M cacodylate buffer and postfixed in buffered 1% osmium tetroxide (OsO4) for 1 hour. After staining with 1% uranyl acetate for 1 hour, the materials were dehydrated in an ethanol series and embedded in LR White resin (medium grade). Thin sections (70–90 nm) were stained with lead citrate, and observed and photographed with a JEOL 100 X transmission electron microscope.
Nuclear DNA contents were measured and normalized to a level of 32C for wild-type trichome nuclei essentially as described previously [28, 29], except that samples were observed with the 200 × objective of a Leica DM RXA2 microscope, and images were captured with a SensiCam QE 12-bit, cooled CCD camera and analyzed with Slidebook software from 3I. Care was taken when setting image capture parameters that the nuclei with the highest DNA content in a group of samples did not saturate the dynamic range of the images. Non-parametric statistics (Kruskal-Wallis One Way ANOVA and Dunn's all pairwise multiple comparison) were performed using SigmaStat. Birefringence was examined using a Nikon FXA microscope equipped with a SpotCam. Samples were cleared with 95% ethanol and placed on a circular rotating stage between two polarizing filters, the polarizer and the analyzer, that were oriented at right angles to each other.
Bowling SA, Clarke JD, Liu Y, Klessig DF, Dong X: The cpr5 mutant of Arabidopsis expresses both NPR1-dependant and NPR1-independent resistance. Plant Cell. 1997, 9: 1573-1584.
Boch J, Verbsky ML, Robertson TL, Larkin JC, Kunkel BN: Analysis of resistance gene-mediated defense responses in Arabidopsis thaliana plants carrying a mutation in CPR5. Mol Plant-Microbe Interact. 1998, 11: 1196-1206.
Kirik V, Bouyer D, Schobinger U, Bechtold N, Herzog M, Bonneville JM, Hulskamp M: CPR5 is involved in cell proliferation and cell death control and encodes a novel transmembrane protein. Curr Biol. 2001, 11 (23): 1891-1895.
Yoshida S, Ito M, Nishida I, Watanabe A: Identification of a novel gene HYS1/CPR5 that has a repressive role in the induction of leaf senescence and pathogen-defence responses in Arabidopsis thaliana. Plant J. 2002, 29 (4): 427-437.
Hülskamp M, Miséra S, Jürgens G: Genetic dissection of trichome cell development in Arabidopsis. Cell. 1994, 76: 555-566.
Melaragno JE, Mehrota B, Coleman AW: Relationship between endoploidy and cell size in epidermal tissue of Arabidopsis. The Plant Cell. 1993, 5: 1661-1668.
Larkin JC, Brown ML, Schiefelbein J: How Do Cells Know What They Want To Be When They Grow Up? Lessons from Epidermal Patterning in Arabidopsis. Annu Rev Plant Biol. 2003, 54: 403-430.
Mathur J: Cell shape development in plants. Trends Plant Sci. 2004, 9 (12): 583-590.
Esch JJ, Chen M, Sanders M, Hillestad M, Ndkium S, Idelkope B, Neizer J, Marks MD: A contradictory GLABRA3 allele helps define gene interactions controlling trichome development in Arabidopsis. Development. 2003, 130 (24): 5885-5894.
Larkin JC, Brown ML, Churchman ML: Insights into the endocycle from trichome development. Cell Cycle Control and Plant Development. Edited by: Inzé D. Blackwell; 2007:249-268.
Perazza D, Herzog M, Hulskamp M, Brown S, Dorne AM, Bonneville JM: Trichome cell growth in Arabidopsis thaliana can be derepressed by mutations in at least five genes. Genetics. 1999, 152 (1): 461-476.
Potikha T, Delmer DP: A mutant of Arabidopsis thaliana displaying altered patterns of cellulose deposition. The Plant Journal. 1995, 7: 453-460.
Updegraff DM: Semi-micro determination of cellulose in biological materials. Anal Biochem. 1969, 32: 420-424.
Schellmann S, Schnittger A, Kirik V, Wada T, Okada K, Beermann A, Thumfahrt J, Jurgens G, Hulskamp M: TRIPTYCHON and CAPRICE mediate lateral inhibition during trichome and root hair patterning in Arabidopsis. Embo J. 2002, 21 (19): 5036-5046.
Schnittger A, Weinl C, Bouyer D, Schobinger U, Hulskamp M: Misexpression of the cyclin-dependent kinase inhibitor ICK1/KRP1 in single-celled Arabidopsis trichomes reduces endoreduplication and cell size and induces cell death. Plant Cell. 2003, 15 (2): 303-315.
Somerville C, Bauer S, Brininstool G, Facette M, Hamann T, Milne J, Osborne E, Paredez A, Persson S, Raab T, Vorwerk S, Youngs H: Toward a systems approach to understanding plant cell walls. Science. 2004, 306 (5705): 2206-2211.
Ahn JW, Verma R, Kim M, Lee JY, Kim YK, Bang JW, Reiter WD, Pai HS: Depletion of UDP-D-apiose/UDP-D-xylose synthases results in rhamnogalacturonan-II deficiency, cell wall thickening, and cell death in higher plants. J Biol Chem. 2006, 281 (19): 13708-13716.
Ellis C, Karafyllidis I, Wasternack C, Turner JG: The Arabidopsis mutant cev1 links cell wall signaling to jasmonate and ethylene responses. Plant Cell. 2002, 14 (7): 1557-1566.
Clarke JD, Volko SM, Ledford H, Ausubel FM, Dong X: Roles of salicylic acid, jasmonic acid, and ethylene in cpr-induced resistance in arabidopsis. Plant Cell. 2000, 12 (11): 2175-2190.
Arioli T, Peng L, Betzner AS, Burn J, Wittke W, Herth W, Camilleri C, Hofte H, Plazinski J, Birch R, Cork A, Glover J, Redmond J, Williamson RE: Molecular analysis of cellulose biosynthesis in Arabidopsis. Science. 1998, 279 (5351): 717-720.
Fagard M, Desnos T, Desprez T, Goubet F, Refregier G, Mouille G, McCann M, Rayon C, Vernhettes S, Hofte H: PROCUSTE1 encodes a cellulose synthase required for normal cell elongation specifically in roots and dark-grown hypocotyls of Arabidopsis. Plant Cell. 2000, 12 (12): 2409-2424.
Brininstool G: A role for CPR5 in promoting cell expansion in Arabidopsis thaliana. Biological Sciences. Baton Rouge , Louisiana State University; 2003:95-
Brown MS, Ye J, Rawson RB, Goldstein JL: Regulated intramembrane proteolysis: a control mechanism conserved from bacteria to humans. Cell. 2000, 100 (4): 391-398.
Hoppe T, Rape M, Jentsch S: Membrane-bound transcription factors: regulated release by RIP or RUP. Curr Opin Cell Biol. 2001, 13 (3): 344-348.
Lusk CP, Blobel G, King MC: Highway to the inner nuclear membrane: rules for the road. Nat Rev Mol Cell Biol. 2007, 8 (5): 414-420.
Larkin JC, Walker JD, Bolognesi-Winfield AC, Gray JC, Walker AR: Allele-specific interactions between ttg and gl1 during trichome development in Arabidopsis thaliana. Genetics. 1999, 151: 1591-1604.
Kirik V, Schnittger A, Radchuk V, Adler K, Hulskamp M, Baumlein H: Ectopic expression of the Arabidopsis AtMYB23 gene induces differentiation of trichome cells. Dev Biol. 2001, 235 (2): 366-377.
Churchman ML, Brown ML, Kato N, Kirik V, Hulskamp M, Inze D, De Veylder L, Walker JD, Zheng Z, Oppenheimer DG, Gwin T, Churchman J, Larkin JC: SIAMESE, a plant-specific cell cycle regulator, controls endoreplication onset in Arabidopsis thaliana. Plant Cell. 2006, 18 (11): 3145-3157.
Walker JD, Oppenheimer DG, Concienne J, Larkin JC: SIAMESE, a gene controlling the endoreduplication cell cycle in Arabidopsis thaliana trichomes. Development. 2000, 127 (18): 3931-3940.
The authors wish to acknowledge the expert assistance of Ying Xiao and Alex Hellman for TEM, David Burk and Ron Bouchard for light microscopy, and M. Cindy Henk for SEM. We also wish to acknowledge Dr. Jim Moroney and Dr. Kirsten Prüfer for critical reading of the manuscript. This work was supported by National Science Foundation Grant IOB 0444560 and the Louisiana Governor's Biotechnology Initiative.
GB backcrossed the cpr5 alleles, generated the cpr5 try double mutants, carried out the cell wall biochemistry, did much of the electron microscopy, prepared several figures, and drafted the manuscript. RK did the scanning electron microscopy of the pGL2:GL3-containing lines and contributed to the DNA content determinations. LAS did the DNA content determinations and statistical analysis, and prepared the final versions of the figures. VK constructed the pGL2:GL3 cpr5-1 line and did the initial analysis on this line, and contributed to drafting the manuscript. MH was involved in the design of initial studies with the pGL2:GL3 cpr5-1 line and helped with the manuscript. JCL participated in the design of the study, did the work on birefringence of cpr5 trichomes, and helped draft the manuscript. All authors have read and approved the final manuscript.
Authors’ original submitted files for images
About this article
Cite this article
Brininstool, G., Kasili, R., Simmons, L.A. et al. Constitutive Expressor of Pathogenesis-Related Genes5affects cell wall biogenesis and trichome development. BMC Plant Biol 8, 58 (2008) doi:10.1186/1471-2229-8-58
- Pathogen Response
- Cell Wall Biogenesis
- Trichome Development
- Trichome Cell
- Putative Membrane Protein