Table 1 Expenditure of time for image acquisition

Action

1st pass: preview scan (5×-objective, 432 × 342 pixels)

2nd pass: detailed scan (10×-objective, 1300 × 1030 pixels)

stage positioning

≈ 0.5 s

≈ 1 s

auto focus

negligible (once per slide)

≈ 3 s

single shot

≈ 0.7 s

≈ 1.5 s

several focal layers

--

5 layers per position

time expenditure

≈ 10 min per slide (scanning 15 × 38 = 570 positions)

≈ 20 min per slide (scanning ≈ 100 positions*)