Figure 8

Indirect immunofluorescence detection of xyloglucan and pectic HG epitopes in TS tobacco stem internode showing higher magnification micrographs of region of cortical collenchyma/parenchyma. a. LM15 binding to cell walls after pectate lyase pretreatment is most strong at outer regions adjacent to middle lamellae. b. Same section as a shown combined with Calcofluor White fluorescence. c. Equivalent section to a immunolabelled with JIM5 showing HG across cell walls and abundance at inner cell walls and intercellular spaces of parenchyma (bottom of micrograph). d. Same section as c shown combined with Calcofluor White fluorescence. Arrows in a and b indicate outer cell wall regions adjacent to middle lamellae with abundant LM15 epitope. Arrows in c and d indicate inner edge of cell walls and abundant JIM5 epitope. Double headed arrows indicate intercellular spaces. Scale = 10 μm.