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Figure 2 | BMC Plant Biology

Figure 2

From: Loss of the N-terminal domain of chlorophyllide aoxygenase induces photodamage during greening of Arabidopsis seedlings

Figure 2

Expression levels of CAO in the etiolated and greening seedlings. A: The expression levels of internal CAO gene and GFP-CAO transgenes in 4-day-old etiolated seedlings and greening seedlings were determined by semi-quantitative PCR. Expression levels from the tubulin (TUB2) gene were monitored as a quantitative control. The internal CAO expression level increased in wild type and ch1-1 seedlings during greening. The CAO transcript of ch1-1 has a 31 bp deletion and it was not detected in the tGABCch and tGBCch plants. B: The accumulation levels of the CAO proteins and GFP-CAO fusion proteins in 4-day-old etiolated seedlings and in greening seedlings were determined. The estimated molecular size of the full-length CAO, GFP-ABC and GFP-BC proteins without transit peptide were 56, 83 and 70 kDa, C: The accumulation levels of the GFP-BC proteins in 4-day-old etiolated tGBCch seedlings during a 24 h greening period were determined. The full-length CAO or GFP-CAO fusion proteins were not detected in wild type, ch1-1 and tGABCch seedlings (data not shown).

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