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Table 1 Total protochlorophyllide accumulation in etiolated seedlings.

From: Loss of the N-terminal domain of chlorophyllide aoxygenase induces photodamage during greening of Arabidopsis seedlings

Strains

Total protochlorophyllide/etiolated seedling (pmole)

Divinyl-protochlorophyllide/total protochlorophyllide (molar ratio)

 

-ALA

+ALA

-ALA

Wild type

0.78 ± 0.02

8.09 ± 1.98

0.37 ± 0.03

tGBCch3

0.33 ± 0.01

2.56 ± 0.56

0.80 ± 0.05

tGABCch3

1.01 ± 0.09

6.29 ± 2.08

0.32 ± 0.03

  1. The seedlings were grown for 6 days under dark conditions with or without ALA (0.5 mg ml-1) on wet filter paper. Seedlings were subsequently harvested under dark conditions and their extracts were analyzed for their protochlorophyllide composition by HPLC analysis (n = 5).
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BMC Plant Biology

ISSN: 1471-2229

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