Figure 2
From: The effects of enhanced methionine synthesis on amino acid and anthocyanin content of potato tubers
Molecular characterisation of transgenic lines. RNA- and protein-blot analysis of the CgSΔ90 transgenic lines C1, C2, C3, and CgSΔ90+zein double-transgenic lines C+Z1 and C+Z2. C, CgSΔ90; Z, zein; D, non-transformed control Désirée; R, ethidium-bromide-stained rRNA bands shown as loading controls. To detect CgSΔ90 and the 15-kD β-zein, proteins of the water-soluble and the ethanol-soluble fractions, respectively, were separated by SDS-PAGE and analysed using antibodies against the 3HA peptide. Molecular mass standards were included in the gels, and the sizes of the relevant markers are indicated in kDa.