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Table 1 Expectations of results from further sequencing of a blackberry (Rubus L.) EST library.

From: A blackberry (RubusL.) expressed sequence tag library for the development of simple sequence repeat markers

Subset sequenced

Multiplication rate

Whole library

 

3884

 

18,432

Number of clones

 

77%

  

3000

 

14,237

Number of high quality sequences

 

22%

  

673

 

3,132

Number of SSR primer pairs to test in parental screens

 

30%

  

202

 

940

Number of primer pairs detecting polymorphisms between two genotypes

 

× 1.9

  

384

 

1,786

Number of polymorphisms to try to map

  1. Expectations are based on results from this study of the percentage of high quality sequences obtained, the percentage of those sequences which contained SSRs, the percentage of primer pairs selected to test, the percentage of SSRs that detected polymorphisms, and the average number of polymorphic products generated from each.