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Figure 1 | BMC Plant Biology

Figure 1

From: Expression of cell wall related genes in basal and ear internodes of silking brown-midrib-3, caffeic acid O-methyltransferase (COMT) down-regulated, and normal maize plants

Figure 1

Histochemical characterization of normal F2, AS225 and F2 bm3 maize lines. F2 = normal F2 line; AS225 = COMT-AS line; bm3 = F2bm3 line. Light microscopy observations of transverse sections in bottom part of ear internode in silking +30 day-old plants stained with Maüle reagent (A-B). Light microscopy observations of transverse sections in bottom part of ear internode in silking plants stained with Wiesner reagent (D, F, H). UV illumination of ear internode bottom part in silking plants (E, G, I). Differences in coloration of lignified tissues between F2, AS225 and F2bm3 lines in the presence of Maüle reagent (A-C) are visible. Metaxylem vessels of ear internode transverse sections (D-I) of AS225 and F2bm3 plants also had a modified lignification (arrow). x = xylem; s = sclerenchyma; p = parenchyma; pp = protophloem; px = protoxylem; mx = metaxylem. Magnification bar was 100 μm (A-C) or 50 μm (D-I).

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