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Figure 4 | BMC Plant Biology

Figure 4

From: Head-neck domain of Arabidopsis myosin XI, MYA2, fused with GFP produces F-actin patterns that coincide with fast organelle streaming in different plant cells

Figure 4

Sub-cellular localization of GFP-MYA2-headIQ compared to that of RFP-FABD2 in A. cepa. F-actin-visualization byGFP-MYA2-head-6IQ at 6–8 h (A), 15 h (B) and 28 h (C) after transformation. The expression of GFP alone results in diffusive cytoplasmic signals (inset in C). F-actin-visualization by RFP-FABD2 at 15 h (D), 25 h (E) and 2 d (F) after transformation. Spatial differences in the F-actin net either labelled with GFP-MYA2-head6IQ (G-I) or RFP-FABD2 (J-L). The focus planes of the upper cell surface (gold particles are visible as black dots) shown by DIC images in (G) and (J) compared to the corresponding fluorescent images in (H) and (K). Images in (G, H) and (J-L) have been acquired from the same plane while the image in (I) derives from a plane 5 μm below. Bar for images in (A-F) in (F) = 5 μm, for images in (G-L) in (L) = 10 μm.

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