Figure 10

Fluorescent microscope analysis of Propidium Iodide uptake during the membrane permeabilization assay. (A-C), light microscope images and (D-F) Fluorescent images of untreated F. oxysporum, F. solani and V. dahliae hyphae respectively. (G-I), Light microscope images and (J-L) fluorescent images of Vv-AMP1 treated F. oxysporum, F. solani and V. dahliae hyphae respectively. Fungi were grown for 40 hours in the presence of Vv-AMP1 at peptide concentrations of 6 μg/ml for F. solani, 9.6 μg/ml for F. oxysporum and 1.8 μg/ml for V. dahliae. Afterwards fungal hyphae were stained with Propidium iodide for 10 min, washed with 1XPBS and subjected to fluorescent microscopic analysis. Bar = 50 μm